Pan-organ poly(A) atlas reveals a post-transcriptional regulatory layer independent of RNA abundance - PubMed
3 hours ago
- #poly(A) tail
- #nanopore sequencing
- #post-transcriptional regulation
- The study created a pan-organ atlas of poly(A) tail lengths across 18 mouse organs using full-length nanopore sequencing, totaling 422 million reads.
- Poly(A) tail length landscapes are heterogeneous and organ-specific, ranging from around 45 nucleotides in the pancreas to approximately 180 nucleotides in reproductive tissues.
- Clustering of isoforms based on cross-organ poly(A) dynamics reveals functionally coherent regulatory modules that are statistically orthogonal to those from transcript abundance.
- Poly(A) length co-regulation can predict known functional interactions even among genes with divergent expression patterns.
- The findings establish poly(A) tail dynamics as an independent, functionally coherent regulatory layer in gene expression.