Engineered bacteriophage nanoassemblies in vivo stabilize DC-T cell immune synapse for high-performance influenza vaccination - PubMed
4 days ago
- #bacteriophage
- #influenza vaccine
- #immune synapse
- Engineered bacteriophage nanoassemblies stabilize DC-T cell immune synapse for enhanced influenza vaccination.
- Nanoassemblies deliver antigen-encoding sequences into DC cytoplasm and boost vaccine potency by enhancing IS stability.
- Influenza hemagglutinin stem gene inserted into bacteriophage genome; DC-targeting peptide fused to bacteriophage sidewall.
- Nanoassemblies inhibit ICAM-1 internalization via NF-κB, PI3K-AKT, and RhoA-ROCK pathways, stabilizing immune synapse.
- Immunization with nanoassemblies triggers strong T cell and antibody responses, providing complete protection and long-term immunity in mice.
- Approach can be adapted for vaccine development against other pathogens due to its flexibility and commonality.