Cu2+-chelated epigallocatechin gallate nanoparticle-functionalized hydrogel promotes osteogenesis by inhibiting ferroptosis - PubMed
3 hours ago
- #ferroptosis-inhibition
- #bone-regeneration
- #hydrogel-therapy
- Cu2+-chelated epigallocatechin gallate nanoparticle-functionalized hydrogel (GGBH@EAC) promotes osteogenesis by inhibiting ferroptosis.
- The hydrogel features EGCG-Arg-Cu (EAC) nanoparticles and nano-hydroxyapatite (nHAp) within a dual-network system of gelatin methacryloyl (GelMA), cationic guar gum (CG), and borax.
- EAC inhibits ferroptosis by reprogramming lipid metabolism and restoring redox homeostasis, while nHAp provides essential minerals for bone regeneration.
- The hydrogel is ROS-responsive, enabling controlled nanoparticle release under high-ROS conditions.
- In vitro, GGBH@EAC scavenged ROS, alleviated mitochondrial damage, and promoted monounsaturated fatty acid synthesis, enhancing BMSC osteogenic differentiation.
- Multi-omics analysis revealed the effects were mediated by SCD1-driven lipid metabolic reprogramming and downregulation of NOX1 and CYP.
- In a rat bone defect model, GGBH@EAC achieved near-complete bone repair and vascularization within 8 weeks without systemic toxicity.
- This work provides a translational platform to direct cellular metabolic fate against stress-induced death, unlocking bone regeneration.