E3 ubiquitin ligase NEDD4 inhibits PEDV infection through ubiquitination and degradation of the viral primase NSP8 - PubMed
8 hours ago
- #Ubiquitination
- #NEDD4
- #PEDV
- NEDD4, an E3 ubiquitin ligase, inhibits Porcine Epidemic Diarrhea Virus (PEDV) infection by ubiquitinating the viral primase NSP8, leading to its degradation.
- Ubiquitination targets a conserved lysine (K170) in an NSP8 SPP motif, with NEDD4's C2 domain crucial for antiviral activity and binding to NSP8.
- NEDD4 upregulation during PEDV infection suppresses viral replication; its knockout enhances infection, confirming its role in host defense.
- Ubiquitinated NSP8 is degraded via both NDP52-dependent selective autophagy and the ubiquitin-proteasome system, with NEDD4 also boosting autophagy through beclin-1 upregulation.
- The NEDD4-NSP8 axis represents a potential therapeutic target for antiviral strategies against PEDV and related coronaviruses.