Highly efficient raw starch degradation by a novel dual-function maltogenic debranching enzyme of the intermediate GH13_20 subfamily from Deinococcus radiodurans - PubMed
8 hours ago
- #starch-degradation
- #biotechnology
- #enzyme
- A novel maltogenic debranching amylase (DrMD) from Deinococcus radiodurans was identified.
- DrMD uniquely exhibits both pullulanase and maltogenic amylase activities at a single active site.
- DrMD showed 4-fold higher efficiency in raw starch degradation compared to type I pullulanase.
- The enzyme hydrolyzes the branch point of large amylopectin and cleaves the amylose structure.
- DrMD effectively produces glucose (G1) and maltooligosaccharides (G2 to G5).
- 86.97% of raw starch was degraded into low-molecular-weight glucans (<1 × 10^4 Da).
- DrMD has potential as a versatile enzyme for industrial starch processing.