Modeling lipid homeostasis using stable isotope tracing and flux analysis - PubMed
4 hours ago
- #non-small cell lung cancer
- #metabolic flux analysis
- #lipid homeostasis
- Lipids play a crucial role in cellular compartmentation and membrane signaling, with dysregulation linked to diseases.
- Limited tools exist for quantifying metabolic fluxes across the lipidome.
- The study introduces Lipid Metabolic Flux Analysis (Lipid-MFA) using stable isotope tracing and mass spectrometry.
- Lipid-MFA quantifies fatty acid synthesis, elongation, headgroup assembly, and salvage reactions in biological systems.
- Findings include decreased fatty acid synthase and VLCFA elongation in p53-deficient NSCLC tumors.
- Increased sphingolipid recycling was observed in p53-deficient versus LKB1-deficient NSCLC tumors.
- Lipid-MFA demonstrated unique ceramide trafficking based on n-acyl chain lengths.
- The approach is useful for elucidating molecular mechanisms in lipid homeostasis.