High-throughput phenotyping of microglial membrane Capacitance: Linking label-free dielectric signatures to polarization continuum and phospholipid remodeling - PubMed
4 days ago
- #Microglia
- #Neuroinflammation
- #Bioelectrical marker
- High-throughput phenotyping of microglial membrane capacitance links dielectric signatures to polarization continuum and phospholipid remodeling.
- The study establishes specific membrane capacitance (Csm) as a label-free, continuous metric for microglial polarization states.
- M1-polarized microglia show significantly elevated Csm compared to M2 or resting states, linked to membrane lipid remodeling.
- The dielectric changes are mechanistically tied to an increased lysophosphatidylcholine/phosphatidylcholine ratio (LPC/PC) regulated by Pla2g4a/Lpcat1.
- PPARγ/SIRT1 activators reversibly modulate these dielectric signatures, confirming Csm's sensitivity to membrane reorganization.
- Csm serves as a real-time, single-cell functional metric for microglial polarization, useful for screening neuroimmunomodulators and predicting therapeutic response.