Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo - PubMed
7 hours ago
- #optical clearing
- #live cell imaging
- #fluorescence imaging
- Development of minimally invasive optical clearing media (SeeDB-Live) for live mammalian tissue imaging.
- SeeDB-Live uses spherical polymers with low osmolarity to minimize light scattering without toxicity.
- Compatible with live cells, enabling structural and functional imaging in spheroids, organoids, brain slices, and in vivo mouse brains.
- Minimal impact on neuronal electrophysiology and sensory responses, facilitating deep cortical layer imaging.
- Useful for epifluorescence voltage imaging in acute brain slices and in vivo preparations.
- Patent application filed by S.I. and T.I. related to SeeDB-Live; other authors declare no competing interests.