Identification of a Key Hemagglutinin Mutation Mediating Antibody Escape in Influenza A(H1N1)pdm09 Viruses - PubMed
4 hours ago
- #Mutation K147N
- #Antibody Escape
- #Influenza A
- The study evaluates the neutralizing ability of the broad-spectrum antibody C12H5 against different H1N1 influenza strains from 2009, 2018, and 2023.
- Using machine learning and SHAP analysis on sequence data, the K147 site in the HA protein was identified as the most important potential escape mutation.
- Experimental validation showed that the K147N mutation reduces neutralization by C12H5 by about 6- to 8-fold while enhancing viral replication fitness in cells.
- Structural analysis places K147 near the receptor-binding domain of HA, explaining its role in antibody escape.
- The K147N mutation has been found in circulating 2023 strains, highlighting its significance for updating vaccines and antibody therapies.