Z-DNA formation regulates the totipotent-like state and primes Zscan4-dependent chromatin compartmentalization - PubMed
5 days ago
- #Z-DNA
- #totipotent-like state
- #chromatin compartmentalization
- Z-DNA formation regulates the totipotent-like state and primes Zscan4-dependent chromatin compartmentalization.
- Transition from one-cell to two-cell stage involves activation of embryonic genes, epigenome reprogramming, and nuclear architecture reorganization.
- Two-cell-like cells from mouse embryonic stem cells recapitulate some characteristics of the two-cell stage, including expression of Dux, Zscan4, and MERVL.
- Zscan4 chromatin factors drive interchromosomal and intrachromosomal interactions, forming the Z compartment independently of cohesin and CCCTC-binding factor.
- Z-DNA formation, regulated by polyamine levels, promotes totipotent-like cells and the establishment of the Z compartment.
- The Z compartment features decreased active histone marks and reduced expression of differentiation-associated genes.
- Z-DNA formation has a dual role: initiating zygotic genome activation (ZGA) and guiding genome compartmentalization to safeguard the totipotent-like state.