CytoplastCXCR4: An enucleated self-eliminating and mitochondria delivering strategy for targeted cartilage defect therapy - PubMed
4 hours ago
- #cartilage regeneration
- #mitochondrial transfer
- #acellular therapy
- Developed an acellular delivery vehicle, cytoplastCXCR4, from enucleated CXCR4-transfected MSCs for targeted mitochondria transport to restore mitochondrial quality control in chondrocytes.
- CXCR4 overexpression enhanced migration to inflamed cartilage via the CXCR4-SDF-1α axis, improving homing ability and retention time at damage sites.
- CytoplastCXCR4 treatment improved mitochondrial structure, oxygen consumption, and ATP synthesis in osteoarthritic chondrocytes in vitro, restoring metabolic balance.
- Compared to exosomes, cytoplasts retain cellular bioactivity, including functional organelles and paracrine signaling, and facilitate mitochondrial transfer via vesicles.
- In a rat knee cartilage defect model, cytoplastCXCR4 precisely targeted damaged cartilage and significantly facilitated cartilage repair more effectively than exosomes.