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Inhibiting cGAS-STING to Preserve Mitochondrial-Nuclear Communication and Stemness in Young Tendon Stem Cells: A Hydrogel Strategy against Age-Related Tendinopathy - PubMed

3 hours ago
  • #tendinopathy
  • #hydrogel-therapy
  • #stem-cells
  • Age-related tendinopathy is common in the elderly and linked to low cellular density and poor blood supply in tendons.
  • Key pathological features include accumulation of senescent tendon-derived stem cells (TDSCs), decrease in young TDSCs, and inflammatory imbalance caused by ROS.
  • Impaired mitochondria-nucleus communication is a central mechanism in disease progression.
  • A ROS-responsive dual-targeted hydrogel (P/H@Lipo) loaded with selenium nanocatalysts (HPSe) and STING inhibitor H-151 was developed.
  • The hydrogel releases L-Lipo@H-151 in response to ROS, targeting TDSCs to inhibit the cGAS-STING pathway.
  • HPSe reduces mtDNA leakage and cGAMP production, strengthening the blockade of the cGAS-STING pathway.
  • This process maintains mitochondrial-nuclear communication and preserves stemness in young TDSCs by preventing senescence.
  • HPSe boosts self-renewal and tendinogenic differentiation in young TDSCs by inhibiting the Hippo signaling pathway.
  • The study presents a novel therapeutic paradigm targeting mitochondrial-nuclear communication to combat age-related tendinopathy.