Visualizing suborganellar lipid distribution using correlative light and electron microscopy - PubMed
4 hours ago
- #nanodomains
- #lipid visualization
- #correlative microscopy
- Introduction of Lipid-CLEM workflow for visualizing suborganellar lipid distribution.
- Combines near-native lipid probes and on-section labelling by click chemistry.
- Enables quantification of relative lipid densities in membrane nanodomains.
- Differential partitioning of sphingomyelin observed in early endosome structures.
- Facilitates simultaneous investigation of proteins and lipids in membrane nanodomains.