Integrated single-cell and spatial analysis reveals context-dependent myeloid-T cell interactions in response to immune checkpoint blockade in head and neck cancer - PubMed
3 hours ago
- #immune checkpoint blockade
- #spatial omics
- #head and neck cancer
- Study evaluates myeloid-T cell interactions in HNSCC patients treated with immune checkpoint blockade (ICB) using spatial and single-cell omics.
- Utilizes Visium spatial transcriptomics and CosMx SMI single-cell spatial omics with a 64-plex protein panel and 1000-gene RNA panel.
- Analyzed 522,399 single cells from 23 patients and spatial RNA-seq data from 8 ICB-treated patients.
- Identified context-dependent differences in myeloid-T cell interactions between Responders and Non-Responders.
- Highlighted specific chemokine interactions (CXCL9/10-CXCR3, CXCL16-CXCR6, CCL4/5-CCR5) as markers for ICB response.
- Provides a resource for studying spatial and ligand-receptor interactions in ICB response, emphasizing context specificity.