Integrated small and long RNA sequencing reveals piRNA mediated transposon repression during human oogenesis - PubMed
12 hours ago
- #transposable elements
- #oogenesis
- #piRNA
- piRNAs, especially PIWIL3-associated short piRNAs, are the main small non-coding RNAs during human oogenesis.
- A significant increase in short-piRNAs correlates with a global reduction in transposable element (TE) expression, particularly LINE-1 and endogenous retroviruses (ERVs).
- PIWIL1- and PIWIL2-associated long piRNAs are linked to the downregulation of specific ERV subfamilies.
- Highly productive piRNA clusters show asymmetric antisense insertion bias toward LINE-1 and ERVs, enabling TE family-specific regulation.
- The study provides a single-cell dataset of small and long RNA co-expression in human oocytes, revealing distinct roles of PIWI/piRNA classes in TE repression.