Analysis of HLA genotype in the recipients' different tissues after haploidentical hematopoietic stem cell transplantation - PubMed
7 hours ago
- #HLA genotyping
- #tissue analysis
- #haplo-HSCT
- Peripheral blood samples are commonly used for HLA genotyping due to easy accessibility and high-quality DNA.
- Haploidentical hematopoietic stem cell transplantation (haplo-HSCT) poses challenges for HLA genotyping in disease relapse cases requiring second transplantation.
- This study analyzed HLA genotypes in various tissues (peripheral blood, buccal swab, saliva) of 66 haplo-HSCT recipients.
- Most peripheral blood samples (55/57) showed the donor's HLA genotype, while buccal swabs (54/62) retained the patient's pre-HSCT genotype.
- Saliva samples showed mixed results: 45% retained patient's genotype, 30% exhibited donor's genotype, and chimerism/HLA loss was detected.
- One patient's buccal sample displayed complete donor HLA genotype replacement.
- Findings aid in selecting tissue samples and interpreting HLA genotyping data for post-HSCT patients.