WormTagDB: A Systematic Survey of Endogenously Tagged Proteins in C. elegans and Roadmap Towards the Tagged Proteome - PubMed
3 hours ago
- #C. elegans
- #Endogenous tagging
- #CRISPR-Cas9
- WormTagDB is a systematic survey of endogenously tagged proteins in C. elegans.
- The study reviewed 2,500 articles and identified 778 reporting novel endogenous tags.
- 1,554 unique genes (8% of the proteome) have been endogenously tagged.
- Tagged proteins are enriched in cytoskeletal proteins, transcription factors, and RNA-binding proteins.
- Membrane proteins, metabolic enzymes, and mitochondrial components remain largely untagged.
- WormTagDB provides an interactive resource for tagged alleles and CRISPR guide designs.
- Aims to prevent redundant strain generation and facilitate new knock-in experiments.
- Complete tagging of C. elegans genes could provide a comprehensive visual proteome.