Xiao-Chaihu-Tang preserves intestinal barrier and ameliorates irinotecan-evoked delayed diarrhea by anchoring endogenous tryptophol to modulate inflammation and oxidation dependent on AhR-UGT1A1-micro
a day ago
- #Gut Microbiota
- #Chemotherapy Side Effects
- #Traditional Medicine
- Xiao-Chaihu-Tang (XCHT) is a traditional formula used to treat diarrhea, including chemotherapy-induced delayed diarrhea (DD).
- The study aimed to uncover the mechanisms behind XCHT's efficacy, focusing on endogenous metabolites, pathways, and gut bacteria.
- XCHT and its key component, tryptophol, improved symptoms of DD, such as perianal status, body weight, and colon pathology.
- Metabolomics and network pharmacology identified 33 potential biomarkers and highlighted the role of tryptophan metabolism and oxidative stress.
- Tryptophol reduced inflammation and oxidative stress, enhancing intestinal barrier function via the AhR-UGT1A1 pathway.
- Metagenomic sequencing revealed XCHT and tryptophol normalized gut bacteria like Lactobacillaceae bacterium and inhibited bacterial invasion.
- Molecular studies confirmed strong binding between tryptophol and AhR-UGT1A1, supporting the therapeutic mechanism.
- XCHT's protective effect is mediated through the tryptophol-AhR-UGT1A1-microbiota axis, offering insights for reducing chemotherapy toxicity.