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Microbial metabolite oxindole curbs acute lung injury by suppressing CXCL13 - PubMed

3 hours ago
  • #gut-lung axis
  • #microbial metabolites
  • #acute lung injury
  • The gut-lung axis plays a role in acute lung injury (ALI) and acute respiratory distress syndrome (ARDS), but its mechanisms are not well understood.
  • Untargeted metabolomics showed dysregulation of tryptophan metabolism in ARDS patients compared to healthy controls.
  • High dietary tryptophan intake alleviated ALI severity in mice, while deficiency worsened it, with protection being gut microbiota-dependent.
  • Lactobacillus johnsonii (L. johnsonii) was found to be depleted during ALI, and its supplementation attenuated ALI, but only with sufficient dietary tryptophan.
  • L. johnsonii converts tryptophan into oxindole, which suppresses CXCL13 in pulmonary macrophages via the aryl hydrocarbon receptor-RelA pathway.
  • Both genetic and pharmacological inhibition of CXCL13 improved ALI symptoms.
  • Oxindole and CXCL13 levels correlated with ARDS severity in patients, indicating clinical relevance.
  • The study highlights a microbiota-dependent gut-lung axis mediated by dietary tryptophan-derived oxindole, suggesting potential therapeutic targets.