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Addition of a collagen mimetic peptide to octacalcium phosphate/gelatin composites enhances bone formation by accelerating calcification - PubMed

7 hours ago
  • #bone formation
  • #collagen mimetic peptide
  • #octacalcium phosphate
  • Adding collagen mimetic peptide (CMP) to octacalcium phosphate/gelatin (OCP/Gel) composites accelerates calcification and enhances bone formation.
  • In vitro studies show OCP/3%Gel/CMP increases alkaline phosphatase activity and alters mesenchymal stromal cell gene expression related to cytoskeleton and immunomodulation.
  • Histomorphometry reveals OCP/3%Gel/CMP achieves the highest calcified matrix area (40.0%), with osteoblasts closely associated with crystal nucleation in the Gel/CMP matrix.
  • In vivo rat femoral defect experiments demonstrate OCP/3%Gel/CMP promotes greater osteoblast and osteoclast accumulation and bone formation compared to OCP/3%Gel at 4 weeks.
  • The CMP triple-helix structure induces gelatin matrix calcification, providing nucleation sites for mineralization and boosting the osteogenic potential of OCP/Gel composites.