Same-Slide Spatial Multi-Omics Integration with IN-DEPTH Reveals Tumor Virus-Linked Spatial Reorganization of the Tumor Microenvironment - PubMed
3 hours ago
- #tumor microenvironment
- #spatial multi-omics
- #DLBCL
- IN-DEPTH is a new workflow integrating spatial proteomics and transcriptomics on the same slide without RNA signal loss.
- Spectral Graph Cross-Correlation (SGCC) is a framework developed to integrate proteomic and transcriptomic data, revealing functional state changes across cell populations.
- Applied to diffuse large B-cell lymphoma (DLBCL), IN-DEPTH and SGCC identified tumor microenvironment changes, including immunosuppressive macrophage enrichment and CD4 T-cell dysfunction.
- The study highlights a potential IL-27-STAT3 signaling axis in EBV-positive tumors, suggesting clinically relevant microenvironmental mechanisms.
- IN-DEPTH aims to support scalable spatial multi-omics for robust AI models in cancer research.