aGPCR-HEK: A Stable High-Expression Inducible Mammalian Cell Expression System for Adhesion GPCR Structural Biology Applications - PubMed
7 hours ago
- #Structural Biology
- #aGPCR
- #HEK293S
- aGPCR-HEK is a stable, tetracycline-inducible mammalian cell expression system for adhesion GPCR (aGPCR) structural biology applications.
- The protocol involves lentiviral production, transduction, flow cytometry enrichment, suspension culture adaptation, and large-scale induction for cryo-EM analysis.
- This system yields milligram-scale quantities of correctly folded aGPCR, suitable for structural and biochemical studies.
- Key features include high-level expression of tagged aGPCRs, efficient genomic integration via lentiviral transduction, and flow cytometry enrichment for enhanced yield.
- The method is particularly useful for ADGRL4, an aGPCR implicated in tumors, which is challenging to express in conventional insect cell systems.