Deciphering the metabolic details of L-lysine toxicity in cyanobacteria - PubMed
3 hours ago
- #Cyanobacteria
- #CRISPRi
- #Lysine toxicity
- L-lysine (Lys) inhibits cyanobacterial growth at micromolar concentrations, similar to antibiotics.
- Exposure to Lys in Synechocystis sp. PCC 6803 causes cell enlargement, oxidative stress, and photosynthesis inhibition, leading to growth arrest.
- Metabolomic profiling shows disruptions in peptidoglycan biosynthesis and altered levels of energy metabolites and other amino acids.
- A CRISPRi-based genetic screen identified the Bgt permease system as essential for Lys resistance.
- UirR and genes linked to c-di-AMP signaling are implicated in Lys metabolism, with c-di-AMP playing a role in cell wall homeostasis.
- The study provides insights into cyanobacterial physiology and potential applications of Lys in bloom-control strategies.