Molecular Insights into the Regulatory Mechanisms Mediated by Hypoxia-Conditioned Skeletal Muscle Exosomal miRNAs - PubMed
8 hours ago
- #exosome
- #miRNA
- #hypoxia
- Hypoxia alters skeletal muscle metabolism and function through exosome-mediated microRNA (miRNA) signaling.
- Study profiled exosomal miRNAs from hypoxic human skeletal muscle cells (HSMCs) to explore their roles in hypoxic adaptation.
- HSMCs were cultured under normoxic or hypoxic conditions, and secreted exosomes were isolated for molecular profiling.
- Seventy-four miRNAs were significantly dysregulated under hypoxia (23 upregulated, 51 downregulated).
- Key miRNAs included upregulated hsa-miR-210-3p and downregulated hsa-miR-486-5p, hsa-miR-127-3p, and hsa-miR-126-3p.
- Predicted targets included 451 genes differentially expressed in hypoxic versus normoxic skeletal muscle cells.
- Functional enrichment highlighted cancer-related, MAPK, PI3K-Akt, and HIF-1 signaling pathways, along with muscle differentiation processes.
- Network analysis identified hsa-miR-20a-5p as a central regulatory hub, followed by hsa-miR-24-3p and hsa-miR-152-3p.
- hsa-miR-24-3p showed the strongest disease associations in the miRNA-disease network.
- Findings suggest exosome-mediated miRNA signaling contributes to hypoxia-driven muscle adaptation and intercellular communication.