Spatiotemporal multiomics reveal a default CD4 fate and a stem-like CD8 T cell subset in the thymus - PubMed
7 hours ago
- #T cell development
- #Thymus
- #Single-cell multiomics
- αβ and γδ T cell lineages diverge as early as the DN1 stage in thymic development.
- CD4 lineage commitment is a transcriptional default program initiated by Gata3, independent of class II MHC signaling.
- CD8 specification requires Runx3 activation and class I MHC signaling, fitting a sequential reprogramming model.
- A stem-like CD8-positive population with self-renewal and developmental potential genes was identified.
- Spatial mapping shows medullary enrichment of CD4 cells and thymocyte exit cues at the cortico-medullary junction.