Mechanisms of topical Mahoniae Caulis against acute mastitis: Integrating SMR, network pharmacology, molecular docking, and experimental validation - PubMed
4 hours ago
- #Mahoniae Caulis
- #NF-κB pathway
- #acute mastitis
- Mahoniae Caulis (MC) is traditionally used in Chinese medicine to treat acute mastitis ('Ru Yong').
- The study used UHPLC-QE-MS to analyze MC's composition and confirmed batch-to-batch consistency.
- Network pharmacology and SMR analysis identified NF-κB signaling as a key therapeutic target.
- Topical MC application reduced inflammation in LPS-induced mastitis in mice, lowering IL-1β, IL-6, and TNF-α levels.
- Molecular docking showed MC's compounds bind effectively to cGAS-STING-NF-κB pathway proteins.
- MC's anti-inflammatory effects are mediated by inhibiting the cGAS-STING-NF-κB pathway.