Precise, minimally evolved adenine base editors generated through mutation reversion analysis - PubMed
3 hours ago
- #TadA
- #ABEs
- #gene-editing
- Adenine base editors (ABEs) enable A•T to G•C base pair changes in the genome.
- Initial ABE development involved directed evolution, introducing 14 mutations into TadA to create ABE7.10.
- Mutation reversion analysis categorized mutations as critical, dispensable, or host-dependent.
- Up to five mutations can be reverted to wild type, creating minimally evolved ABEs (ME-ABEs).
- ME-ABEs show narrow editing windows, enhanced on-target editing, and low off-target activity.
- ME-ABEs efficiently edit six clinically challenging sites that were problematic for ABE7.10, ABE8e, or ABE8.20.
- A.C.K. has disclosed conflicts of interest related to patents and equity in biotech companies.