mRNA-based CAR T cell engineering: Unmodified mRNA enables high CAR expression without innate immune activation in T cells - PubMed
3 hours ago
- #immune activation
- #CAR T cells
- #mRNA
- Unmodified mRNA enables high CAR expression in T cells without triggering innate immune activation.
- T cells uniquely do not activate immune responses to unmodified mRNA, unlike other cell types tested.
- The absence of nucleotide modifications in mRNA enhances CAR expression and cytotoxic potency in T cells.
- Findings suggest mRNA can be a non-inflammatory, efficient tool for T cell engineering, reducing manufacturing costs.
- The study challenges the current gold standard of using modified nucleosides like N1-methylpseudouridine in mRNA applications.