Combined Patch-clamp Electrophysiology and Single-Cell Genomic Analysis Reveal Spiking Tumor Cells at the Neocortical Glioblastoma Interface in Humans - PubMed
4 hours ago
- #Patch-seq
- #Glioblastoma
- #Cancer Neuroscience
- Combined patch-clamp electrophysiology and single-cell genomic analysis used to study glioblastoma cells (GBCs) at the neocortical leading edge.
- More than half of leading edge cells show aberrant action potentials (aAPs), resembling neurodevelopmental cells.
- Reconstructed cells exhibit abnormal somatodendritic morphology.
- GBCs and non-tumor cells share similar electrophysiological phenotypes, including aAP generation and depolarized membrane potential.
- Transcriptomic analysis reveals aAP phenotype across diverse GBC states, correlating with lower proliferation-related pathways but higher inflammatory/immune, angiogenic, and mesenchymal transition pathways.
- Non-tumor cells show hybrid transcriptomic signatures with predominantly neuronal and minor astrocytic features.
- Findings support the hypothesis of neuronal mimicry across different glioma types.