Single-cell transcriptomics reveals heterogeneity and immune microenvironment in lymphatic metastasis of head and neck squamous cell carcinoma - PubMed
5 hours ago
- #single-cell transcriptomics
- #lymphatic metastasis
- #HNSCC
- Single-cell transcriptomics used to study lymphatic metastasis in head and neck squamous cell carcinoma (HNSCC).
- Comparative scRNA-seq analysis performed on seven metastatic and five non-metastatic lymph node tissues.
- Identified altered cell types: CD8+ Tex cells, Macrophages, and Cancer-associated fibroblasts (CAFs).
- Tumor cells classified into seven clusters, with cluster2 linked to tumorigenesis and metastasis.
- Specific subsets (e.g., CD4_Tfh_CXCL13, CD8_teff_CREM) associated with HNSCC progression and metastasis.
- Prognostic model constructed using INHBA, SFRP2, SPP1, and IFI27 for risk stratification and therapy.