Multiomic single-cell perturbation screens reveal critical lncRNA regulators of senescence - PubMed
4 hours ago
- #senescence
- #CRISPR-screening
- #lncRNA
- Systematic perturbation screens identified critical long noncoding RNAs (lncRNAs) regulating senescence using a CRISPR-dCas9-KRAB knockdown system integrated with single-nucleus multiomics profiling.
- HOTAIRM1, a DNA repair-associated lncRNA, stabilizes DNA repair by cooperating with BANF1 and p53 at double-strand break loci within condensates; its deficiency triggers p53-mediated senescence.
- In aged mouse lungs, adeno-associated virus-mediated overexpression of HOTAIRM1 reduced fibrosis, alleviated tissue damage, and promoted cellular proliferation, highlighting its therapeutic potential for age-related diseases.