Scalable and multimodal brain angiogenesis and blood-brain barrier genetics by somatic mutagenesis - PubMed
4 hours ago
- #CRISPR-Cas9
- #blood-brain barrier
- #angiogenesis
- The study presents a somatic CRISPR-Cas9 gene disruption strategy to investigate brain angiogenesis and blood-brain barrier (BBB) permeability.
- The approach leverages the advantages of both mice and zebrafish for rapid phenotypic assessment within two to six weeks.
- Brain angiogenesis is studied in transparent embryonic zebrafish hindbrain, while BBB maintenance is evaluated in endothelial-specific Cas9 transgenic mice.
- The method enables multimodal BBB functional genomics with quick turnaround time and multiplexing capacity.
- The research addresses the limitations of current in vitro and in vivo models for studying BBB ontogeny and regulation.