Single-cell multiomic atlas of healthy pediatric bone marrow reveals age-dependent differences in lineage differentiation driven by stromal signaling - PubMed
3 days ago
- #single-cell atlas
- #hematopoiesis
- #bone marrow
- Study presents a single-cell multiomic atlas of healthy pediatric bone marrow.
- Analyzes mRNA and surface protein expression in 90,710 cells, including HSPCs and MSCs from 9 donors (ages 2-32).
- Young pediatric (YP) bone marrow (<10 years) differs from adolescent/young adult (AYA) bone marrow (≥13 years).
- Hematopoietic output shifts from B cell dominance in YP to myeloid/T cell bias in AYA.
- Spatial transcriptomics confirms age-dependent changes in bone marrow composition.
- Two lymphoid progenitor (LyP) subsets regulate lineage shift: CD127+ LyP (B cell-biased) in YP, CD127- LyP (lymphoid/myeloid) in AYA.
- Stromal signaling changes with age, with higher IL-7 production by MSCs in YP.
- Findings suggest niche-mediated regulation of HSPC lineage potential during development.
- Provides a resource for understanding hematopoietic development and early-life origins of hematologic disease.