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Scalable assay to identify inhibitors of prion-like propagation of protein misfolding as potential therapeutics for neurodegeneration - PubMed

4 hours ago
  • #protein-misfolding
  • #drug-discovery
  • #neurodegeneration
  • Protein misfolding is linked to neurodegenerative diseases and can propagate via a prion-like mechanism.
  • A scalable enzymatic assay was developed to test inhibitors of prion-like conversion in SOD1, linked to ALS.
  • Some small-molecule inhibitors like telbivudine and cisplatin delayed conversion, while others like baicalein and quercetin had little effect.
  • Surprisingly, statins accelerated conversion, potentially promoting ALS progression.
  • The assay distinguishes between conversion and aggregation, offering promise for future drug discovery targeting misfolding propagation.