A stable all-D peptide ligand inspires broad-spectrum affinity and high performance in the purification of COVID-19 vaccines - PubMed
3 days ago
- #COVID-19 Vaccine
- #Affinity Chromatography
- #D-peptide Ligand
- A stable all-D peptide ligand (D-HWK) was developed for broad-spectrum affinity in COVID-19 vaccine purification.
- D-HWK showed higher affinities for wild-type RBD, trimer S protein, and RBDs from variants of concern compared to its L-enantiomer (L-HWK).
- Molecular dynamics simulations revealed D-HWK had a more stable binding conformation due to enhanced π-π stacking and hydrogen bond networks.
- Sep6FF-d-HWK chromatography demonstrated broader binding conditions and efficient elution under mild conditions.
- Seng4FF-d-HWK chromatography successfully purified wild-type COVID-19 and Omicron vaccines with high stability over 20 purification cycles.
- D-HWK exhibited great tolerance to enzymatic hydrolysis, improving purification performance and stability.
- The research provides a strategy for accelerating affinity chromatography development for future pandemics.