High-resolution in situ structures of hantavirus glycoprotein tetramers - PubMed
3 hours ago
- #glycoprotein
- #cryo-EM
- #hantavirus
- High-resolution in situ structures of hantavirus glycoprotein tetramers were determined using a cryo-electron microscopy workflow.
- The study revealed previously uncharacterized features of glycoprotein organization, stability, and pH sensing.
- A 2.35 Å resolution structure of the membrane-embedded Andes virus (ANDV) glycoprotein tetramer was established.
- Structures of dimers of tetramers and a complex with antibody ADI-65534 were also determined.
- Immunization of mice with self-amplifying replicon RNA (repRNA) encoding ANDV-VLPs elicited high levels of glycoprotein-binding antibodies.
- The findings advance understanding of hantavirus glycoprotein assemblies and support structure-based vaccine design.