The EP424R protein of African swine fever virus functions as a 2'-O-methyltransferase and plays an important role in viral replication - PubMed
5 days ago
- #2'-O-methyltransferase
- #African swine fever virus
- #viral replication
- The EP424R protein of African swine fever virus (ASFV) functions as a 2'-O-methyltransferase (2'-O-MTase).
- EP424R plays a critical role in viral replication by modifying viral mRNA with a cap-1 structure (m7GpppNmp-RNA).
- Crystal structures of EP424R with cofactors (SAM, SAH, SFG) were determined, revealing key catalytic residues (KDKE).
- Overexpression and siRNA experiments confirmed EP424R's importance in ASFV replication, partially dependent on its methyltransferase activity.
- EP424R is a potential antiviral target due to its role in ASFV mRNA capping and replication.