Purification of post-transcriptionally modified tRNAs for enhanced cell-free translation systems - PubMed
7 hours ago
- #cell-free translation
- #post-transcriptional modifications
- #tRNA purification
- Purification method developed for post-transcriptionally modified tRNAs to enhance cell-free translation systems.
- Method couples tRNA overexpression with DNA hybridization-based purification, producing high-yield, pure tRNAs with native modifications.
- Technique applied to Mj-tRNA_{CUA}^{Opt} and Ma-tRNA_{CUA}^{Pyl}, confirming native E. coli modifications and improved amber codon suppression.
- Engineered variant of E. coli tRNA_{CCA}^{Trp} also purified, demonstrating method's applicability to synthetic tRNAs.
- Addresses a critical challenge in RNA biochemistry by generating modified tRNAs efficiently.