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Een inkijkje in de broncode van het BioNTech/Pfizer SARS-CoV-2-vaccin - Bert Hubert's writings

a day ago
  • The mRNA vaccine contains genetic code that instructs cells to produce the SARS-CoV-2 spike protein.
  • The mRNA is modified with pseudouridine (1-methyl-3'-pseudouridylyl) to evade immune detection and enhance stability.
  • The code includes a 5' cap, optimized 5' and 3' untranslated regions (UTRs), a signal peptide, and a codon-optimized spike protein sequence.
  • Two proline substitutions in the spike protein stabilize it in the prefusion conformation, improving immune response.
  • Codon optimization increases G and C content for higher protein expression efficiency.
  • The poly-A tail has a unique linker sequence, possibly for further optimization.
  • The vaccine leverages decades of research and is 95% effective.